Reversible conformational change of cytochrome c at a modified gold electrode in methanol.
نویسندگان
چکیده
Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.
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ورودعنوان ژورنال:
- Physical chemistry chemical physics : PCCP
دوره 12 34 شماره
صفحات -
تاریخ انتشار 2010